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目的探讨利用HER-2阳性细胞系SKBr-3建立自发性乳腺癌肝转移模型,为乳腺癌肝转移的机制及治疗学研究提供有价值的模型。方法利用SKBr-3乳腺癌细胞株接种至16只裸鼠第二对乳腺脂肪垫,建立裸鼠第一代原位乳腺癌模型,再利用建立成功的原位肿瘤组织在16只裸鼠原位进行瘤组织块第二代原位种植,最后利用第二代组织块种植成功的瘤组织对16只裸鼠再次进行原位肿瘤种植,分别观察肿瘤肝转移情况。结果在第一代肿瘤细胞原位种植的肿瘤中,没有发生肝转移现象。而在第二代组织原位种植瘤中,只有1只发生肝转移,而在第三代的组织原位肿瘤种植模型中出现了接近81.25%(13/16)的肝转移。无一只出现肉眼肺转移。结论乳腺癌裸鼠体内反复传代可以建立高效、相对特异性的自发性乳腺癌肝转移模型,为肝转移机制及治疗研究提供很好的模型。
Objective To explore the use of HER-2 positive cell line SKBr-3 to establish a spontaneous breast cancer model of liver metastasis and to provide a valuable model for the mechanism of liver metastasis and therapeutics of breast cancer. Methods The first generation of breast cancer in nude mice model was established by inoculating SKBr-3 breast cancer cell line with the second pair of mammary fat pads in 16 nude mice. The established in situ tumor tissue The second generation of tumor tissue was planted in situ. Finally, 16 nude mice were reimplanted in situ with tumor tissue successfully planted by the second generation of tissue mass to observe the liver metastasis. Results In the first generation of tumor cells in situ implantation of the tumor, no liver metastasis. In the second generation of orthotopic implants, only one developed liver metastases, whereas nearly 81.25% (13/16) of liver metastases occurred in the third generation of orthotopic tumor in situ models. None of the naked eye lung metastases. Conclusion Repeated passage in breast cancer nude mice can establish a highly efficient and relatively specific model of spontaneous liver metastasis of breast cancer and provide a good model for liver metastasis mechanism and treatment research.