本文应用基因工程技术构建了可溶性H 2Kb 分子与绿色荧光蛋白 (GFP)融合基因sKb GFP。在COS7细胞中表达后 ,在内质网区域呈现明显绿色荧光。RT PCR结果显示融合基因获得了正确表达 ,蛋白印迹分析表明细胞内产生了预期大小的融合蛋白。细胞培养上清经亲和层析柱纯化后 ,获得了预期大小的重组蛋白。 In this paper, soluble h 2Kb molecule and green fluorescent protein (GFP) fusion gene sKb GFP were constructed by genetic engineering. After expressed in COS7 cells, there was a clear green fluorescence in the endoplasmic reticulum. RT PCR results showed that the fusion gene was correctly expressed, and Western blot analysis showed that a fusion protein of the expected size was produced in the cell. After the cell culture supernatant was purified by affinity chromatography, the recombinant protein of the expected size was obtained.