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已有许多报导指出,职业性苯接触会引起周围淋巴细胞和骨髓细胞中的染色体畸变。而环境中的诱变剂或致断裂剂(clastogenic agent)的协同作用的可能性正在增加。为此,作者观察了在离体条件下苯和辐射对培养53小时的人体周围血淋巴细胞染色体畸变的协同作用。在正常成年男性外围血(约0.3 ml)的培养液中,分别加入浓度为4.0×10~(-5)、2.0×10~(-4)及1.0×10~(-8)M苯,以不加苯者为对照,另一组在加苯后用总剂量为100拉德的γ-射线处理,以不含苯但经射线处理过的为对照。培养后作Giemsa染色,显微照相后记录染色体结构畸变,包括双着丝点、环、无着丝点断片、断裂和裂隙(包括染
It has been reported in many reports that occupational benzene exposure causes chromosomal aberrations in the surrounding lymphocytes and myeloid cells. The potential for synergistic mutagens or clastogenic agents in the environment is increasing. To this end, the authors observed the synergistic effect of benzene and radiation on chromosome aberrations in human peripheral blood lymphocytes cultured for 53 hours under ex vivo conditions. Concentrations of 4.0 × 10 -5, 2.0 × 10 -4 and 1.0 × 10 -8 M were added to normal adult male peripheral blood (about 0.3 ml) Benzene was not added as a control, and the other group was treated with γ-rays with a total dose of 100 rad after addition of benzene, with no benzene but radiation-treated controls. Giemsa staining was performed after culturing, and chromosomal structural aberrations were recorded after photomicrography, including dicentrics, rings, no-filament fragments, breaks and fissures (including staining