细胞的兴奋、收缩与分泌等功能依赖于膜内外离子交换,现已证明细胞膜上离子通道(通常为一蛋白分子)正常的活动乃是这一交换的重要保证。离子通道活动异常便导致疾病。因此,研究细胞膜离子单通道的活动规律是当前医学分子生物学的一个新发展。电生理学的最新技术膜片电压固定法为直接观察这一活动规律提供了方法。目前我们已建立了记录离子单通道电活动的实验室,改进了膜片电压固定实验技术,在以下两方面开展了工作。第一,结合关于血压调节与高血压发病机制的研究,探讨小动脉平滑肌的收缩,挛缩机制与钙、钾等离子通道活动的关系。已观察到血压正常的Wistar大鼠肠系膜小动脉(口径为 Functions such as excitability, contraction and secretion of cells depend on ion exchange both inside and outside the membrane. The normal activity of ion channels (usually a protein molecule) on the cell membrane has been shown to be an important guarantee of this exchange. Abnormal ion channel activity leads to disease. Therefore, the study of the activity of single channel of membrane ions is a new development in current medical molecular biology. The latest technology in electrophysiology Diaphragm voltage fixation provides a direct method of observing this pattern of activity. At present, we have set up a laboratory that records the single-channel ionization activity of ions and has improved the experimental technique of fixing the voltage of the diaphragm, and has carried out the work in the following two aspects. First, combined with the research on the pathogenesis of hypertension and hypertension, the relationship between contractile and contracture mechanisms of the arteriolar smooth muscle and the activity of calcium and potassium channels was explored. A normotensive Wistar rat mesenteric arteriole has been observed (caliber