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目的:建立兔单侧肺血管阻断后再开放模型,用于探讨肺外科中肺缺血-再灌注损伤的特点。方法:同种新西兰白兔96只,随机分成3组,Ⅰ组为对照组(n=36);Ⅱ组为单纯阻断肺动脉组(n=30);Ⅲ组为阻断肺循环组(n=30)。术中监测动物血流动力学指标;分别于开胸时(仅Ⅰ组)、缺血1h和再灌注1、2、4、6h测定左肺静脉血氧分压;检测肺组织中丙二醛(MDA)含量;取肺组织作病理学观察,并测定手术侧肺湿/干比。结果:各组动物术中血流动力学指标平稳;在缺血1h和再灌注1、2、4、6h,Ⅰ组与Ⅱ、Ⅲ组相比,氧分压、肺湿/干重比及肺组织中MDA含量的差异均有显著性(P<0.05和P<0.01),Ⅲ组与Ⅱ组相比,差异无显著性(P>0.05);Ⅱ、Ⅲ组呈现相似的肺损伤病理改变过程,均在再灌注4h出现较为明显的肺损伤,再灌注6h肺损伤呈恢复趋势。结论:该方法的建立为进一步研究肺外科中肺缺血-再灌注损伤提供了较为理想的动物模型。
OBJECTIVE: To establish a model of unilateral pulmonary vascular occlusion in rabbits and then open the model to investigate the characteristics of lung ischemia-reperfusion injury in lung surgery. Methods: Ninety-six New Zealand white rabbits were randomly divided into three groups: control group (n = 36), group Ⅱ (n = 30) 30). Intraoperative monitoring of hemodynamic parameters in animals; respectively left ventricular thoracotomy (only Ⅰ group), ischemia 1h and reperfusion 1, 2, 4, 6h determination of left pulmonary venous oxygen pressure; detection of lung tissue malondialdehyde The content of MDA was measured. The lung tissue was taken for pathological observation and the wet / dry ratio of the lateral lung was measured. Results: The indexes of hemodynamics in each group were stable. The oxygen partial pressure, wet / dry weight ratio of lung and the ratio of lung wet weight (P <0.05 and P <0.01). There was no significant difference between group Ⅲ and group Ⅱ (P> 0.05). The pathological changes of lung injury in group Ⅱ and Ⅲ showed similar During the course of reperfusion, the lung injury was more obvious at 4h after reperfusion, and the lung injury recovered at 6h after reperfusion. Conclusion: The establishment of this method provides an ideal animal model for the further study of lung ischemia-reperfusion injury in lung surgery.