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背景:对于骨形态发生蛋白的生物活性分析中发现,极其微量的骨形态发生蛋白在成年个体中被分泌和储存在骨基质和成骨、成软骨等细胞中,当骨折时这些骨形态发生蛋白在骨折区域释放,诱导周围的间充质细胞发生化学趋化、聚集、分化并形成软骨,从而最终形成成熟的骨结构。目的:验证转基因人骨形态发生蛋白7在兔椎间盘组织中的成骨作用。方法:健康成年新西兰大白兔随机分成4组。分别于不同的腰椎间盘注入骨形态发生蛋白7溶液,生理盐水,或使用金属手术器械行腰椎间盘单纯破坏,并设立未干预的腰椎间盘作为空白对照。术后第6周应用组织学观察注射区内新骨的形成情况。结果与结论:各组动物注射生理盐水的椎间盘和空白椎间盘均为阴性结果。注射骨形态发生蛋白组的新生骨的面积显著大于单纯破坏组(P<0.05)。注射骨形态发生蛋白组新生骨面积组内差异在放大40倍和100倍时,差异无显著性意义(P>0.05)。结果证实转基因人骨形态发生蛋白7能将家兔的椎间盘转化为新生软骨组织。
BACKGROUND: In the bioactivity analysis of bone morphogenetic proteins, it has been found that extremely trace amounts of bone morphogenetic proteins are secreted and stored in adult individuals in the bone matrix and in cells such as osteogenesis, cartilage and the like, and these bone morphogenetic proteins Release in the fracture area induces chemotaxis, aggregation, differentiation and formation of cartilage in the surrounding mesenchymal cells, resulting in the formation of a mature bone structure. OBJECTIVE: To verify the osteogenic effect of transgenic human bone morphogenetic protein 7 in rabbit intervertebral disc tissue. Methods: Healthy adult New Zealand white rabbits were randomly divided into 4 groups. Bone morphogenetic protein 7 solution, saline, or metal surgical instruments were used to perform lumbar intervertebral disc disruption in different lumbar intervertebral discs respectively. Intervertebral discs with no intervertebral disc were set as blank control. Six weeks after operation, histological examination of new bone formation in the injection area was performed. RESULTS AND CONCLUSION: The animals in each group were injected normal saline disc and blank disc were negative results. Bone morphogenetic protein injection of new bone area was significantly greater than the simple destruction group (P <0.05). There was no significant difference in the differences between the groups injected with bone morphogenetic protein in new bone area (P> 0.05). The results confirmed that the transgenic human bone morphogenetic protein-7 can transform the rabbit disc into nascent cartilage tissue.