论文部分内容阅读
为研究靛玉红对ATP引起的巨噬细胞免疫反应的影响,采用中性红摄入法检测细胞吞噬功能,用MTT法检测细胞活力,通过DHE探针检测ROS的产生。结果表明:细胞外ATP可抑制巨噬细胞吞噬、导致细胞死亡并促进ROS产生,靛玉红对ATP引起的以上免疫反应有抑制作用。为了解靛玉红的作用机制,进一步研究其对ATP引起的[Ca2+]i升高和P2X7受体介导的膜通透性增加的影响。结果显示:靛玉红可降低ATP引起的[Ca2+]i升高,抑制ATP诱发的EB进入。结果提示,靛玉红对P2X7的活化有阻断作用,其对ATP引起的免疫反应的抑制效果可能是通过抑制P2X7受体实现的。
In order to study the effect of indirubin on the immune responses induced by ATP in macrophages, phagocytosis was detected by neutral red ingestion, cell viability was assayed by MTT assay, and ROS production was detected by DHE probe. The results showed that: Extracellular ATP can inhibit phagocytosis of macrophages, leading to cell death and promote the production of ROS, indirubin on the ATP-induced immune response was inhibited. In order to understand the mechanism of action of indirubin, we further investigated its effect on ATP-induced increase of [Ca2 +] i and P2X7 receptor-mediated increase of membrane permeability. The results showed that indirubin could reduce the increase of [Ca2 +] i induced by ATP and inhibit the entry of EB induced by ATP. The results suggest that indirubin blocks the activation of P2X7, and its inhibitory effect on ATP-induced immune response may be through inhibition of P2X7 receptor.