轮状病毒是婴幼儿秋季腹泻的重要病原。以前多用电镜检测,但由于其设备昂贵、灵敏度较低,又不能及时地发出诊断报告,因而限制了它的使用。国外相继应用补体结合试验、对流免疫电泳、放射免疫、酶联免疫吸附、固相免疫电镜等方法。本文应用圆片法酶联免疫吸附试验(圆片法EL-ISA)、微板法ELISA、补体结合试验(CFT)和直接电镜(DEM)检测人轮状病毒(HRV),并对其结果进行比较,以探讨一种灵敏、特异、快速、易于推广的检测方法。 Rotavirus is an important pathogen of infantile autumn diarrhea. Previously used electron microscopy, but because of its expensive equipment, low sensitivity, and can not issue a timely diagnostic report, thus limiting its use. Abroad have been applied complement fixation test, convective immunity electrophoresis, radioimmunoassay, enzyme-linked immunosorbent, solid phase immunoelectron microscopy and other methods. In this paper, human rotavirus (HRV) was detected by disk ELISA-EL-ISA, microplate ELISA, complement fixation test (CFT) and direct electron microscopy (DEM) Compared to explore a sensitive, specific, rapid, easy to promote the detection method.