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研究维甲酸受体α（RARα）基因在乳腺癌细胞中的表达情况。用NorthernBlot、WesternBlot和免疫组化等方法。结果：RARα受体蛋白表达与其mRNA表达相一致，RARαmRNA表达在ER阳性乳腺癌细胞中比ER阴性的乳腺癌细胞高，RARα受体蛋白表达也是如此，RARαmRNA及RARα受体蛋白表达都与ER状况相关。结论：3种检测方法都具可信性，实际工作中可以因地制宜选用合适的检测手段为临床服务。
To study the expression of retinoid receptor alpha (RARα) gene in breast cancer cells. NorthernBlot, WesternBlot, and immunohistochemistry were used. RESULTS: RARα receptor protein expression was consistent with its mRNA expression. RARα mRNA expression was higher in ER positive breast cancer cells than ER negative breast cancer cells. The expression of RARα receptor protein was also the same, both RARα mRNA and RARα receptor protein expression were correlated with ER status. Related. Conclusion: The three methods are credible. In practical work, suitable testing methods can be selected for clinical services.