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目的探讨肥胖女童血浆和低密度脂蛋白(LDL)中抗氧化维生素水平的变化。方法随机选取肥胖百分数超过20%的单纯性肥胖女童27例(肥胖组),年龄7～13岁。另取健康对照同龄女童17例为健康对照组。测定各组血浆和LDL中α-生育酚和β-胡萝卜素水平,分析各组LDL的脂肪酸成分,并计算其过氧化应激指数(PI)。α-生育酚和β-胡萝卜素采用装备有电化学检测器的反相高效液相色谱仪测定,LDL中的脂肪酸采用装备有火焰离子化检测器的气相色谱仪测定。应用SPSS 17.0软件进行统计学分析。结果肥胖组血浆中的α-生育酚/总脂为4.36 nmol.mg-1,显著低于健康对照组(5.33 nmol.mg-1)(t=2.71,P<0.05)。LDL中的β-胡萝卜素和α-生育酚水平在肥胖组中分别为0.299 nmol.mg-1和8.77 nmol.mg-1,显著低于健康对照组的0.606 nmol.mg-1和13.14 nmol.mg-1(Pa<0.05)。肥胖组儿童LDL含有更多的硬脂酸、油酸和花生四烯酸,其PI值为83.9,显著高于健康对照组的71.2(P<0.05)。结论肥胖女童存在抗氧化维生素不足、对氧化应激的敏感性增加的现象,这可能是其成年后发生动脉粥样硬化的原因之一。
Objective To investigate the changes of antioxidant vitamins in obese girls with plasma and low density lipoprotein (LDL). Methods Twenty-seven simple obese girls (obese group) with a prevalence of over 20% were randomly selected, aged 7-13 years. Another healthy control girls of the same age 17 cases as healthy control group. The levels of α-tocopherol and β-carotene in the plasma and LDL of each group were determined. The fatty acid composition of LDL of each group was analyzed and its peroxidation stress index (PI) was calculated. Alpha-tocopherol and beta-carotene were determined using an RP-HPLC equipped with an electrochemical detector, and fatty acids in LDL were measured using a gas chromatograph equipped with a flame ionization detector. Application SPSS 17.0 software for statistical analysis. Results The plasma α-tocopherol / total lipid was 4.36 nmol.mg-1 in obesity group, which was significantly lower than that in healthy control group (5.33 nmol.mg-1) (t = 2.71, P <0.05). Β-carotene and α-tocopherol levels in LDL were 0.299 nmol.mg-1 and 8.77 nmol.mg-1 in the obese group, significantly lower than the 0.606 nmol.mg-1 and 13.14 nmol in the healthy controls. mg-1 (Pa <0.05). LDL in obese children contained more stearic acid, oleic acid and arachidonic acid, with a PI of 83.9, which was significantly higher than that of healthy controls (P <0.05). Conclusion Obese girls have the deficiency of antioxidant vitamins and their increased sensitivity to oxidative stress, which may be one of the causes of atherosclerosis in adulthood.