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目的 :探讨复方丹参方对拟血管性痴呆大鼠的治疗作用机理。方法 :Wistar雄性大鼠经夹闭双侧颈总动脉 (CCA) ,并腹腔注射硝普钠 (2 .5 mg· kg- 1 )造模。选术后 7d存活的有显著学习记忆障碍的大鼠 4 0只为血管性痴呆模型 ,随机分为 4组。西药组给予喜得镇悬浮液 (0 .5 mg· kg- 1 · d- 1 ) ,复方丹参大小剂量组给予复方丹参方稀释液 (剂量分别为 10 g· kg- 1 ·d- 1、5 g· kg- 1· d- 1 ) ,模型组与正常组均给予等容量生理盐水灌胃。连续 2 1天。结果 :模型组与正常组比较 ,模型组大鼠脑内 Ach、5 - HT含量明显降低 ,Ach E活性明显升高 (P<0 .0 1)。复方丹参方大剂量组能明显升高 Ach含量及 5 - HT含量 ,降低脑内 Ach E活性 (P<0 .0 5 ,0 .0 1) ;复方丹参方小剂量组亦有类似作用 ,但 P>0 .0 5。与西药组比较无显著差异 (P>0 .0 5 )。结论 :复方丹参方大小剂量均能改善胆碱能系统的功能 ,调节脑组织 Ach生理代谢 ,从而改善记忆
Objective : To explore the therapeutic mechanism of Compound Danshen Prescription on Vascular Dementia Rats. METHODS: Male Wistar rats were clamped on both common carotid arteries (CCA) and intraperitoneally injected with sodium nitroprusside (2.5 mg·kg-1). Seventy-four rats with significant learning and memory impairment who survived 7 days after surgery were vascular dementia models and were randomly divided into 4 groups. Western medicine group was given Xideng town suspension (0.5 mg·kg-1 ·d-1), and compound Danshen large-dose group was given compound Danshen prescription diluent (dose was 10 g·kg-1 ·d-1,5 respectively). g · kg - 1 · d - 1 ) , the model group and the normal group were given equal volume saline. For 2 consecutive days. RESULTS: Compared with the normal group, the Ach and 5-HT levels in the brain of the model group were significantly decreased, and the Ach E activity was significantly increased (P<0.01). Compound Danshenfang large-dose group can significantly increase Ach content and 5-HT content, and reduce Ach E activity in the brain (P<0.05, 0.01); Compound Danshenfang small-dose group also has a similar effect, but P>0. 0 5. There was no significant difference from the western medicine group (P>0.05). Conclusion : Both the doses of compound Danshenfang can improve the function of the cholinergic system and regulate the physiological metabolism of Ach in the brain, thus improving memory.