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目的:研究食管鳞癌(esophageal squamous cell carcinoma,ESCC)与食管腺癌(esophageal adenocarcinoma,EAC)的基因差异表达,探讨ESCC与EAC发生发展的基因学基础。方法:选取8例ESCC和8例EAC组织抽提mRNA,应用cDNA芯片技术通过芯片杂交、生物信息学处理,找出两者间差异表达基因。结果:采用BioStarH-40芯片发现差异表达基因541条,差异表达基因占13.8%,其中表达增强309条(显著增强73条),表达降低232条(显著降低61条)。结论:ESCC与EAC基因表达比较,差异有统计学意义,这些差异可能在两类肿瘤不同的生物学行为中起重要作用。
OBJECTIVE: To study the differential gene expression of esophageal squamous cell carcinoma (ESCC) and esophageal adenocarcinoma (EAC), and to explore the genetics basis of the occurrence and development of ESCC and EAC. Methods: mRNA was extracted from 8 cases of ESCC and 8 cases of EAC tissue. CDNA microarray was used to identify the differentially expressed genes by chip hybridization and bioinformatics analysis. Results: 541 differentially expressed genes were found in BioStarH-40 chip, with 13.8% of differentially expressed genes, of which 309 were significantly enhanced (73 significantly increased) and 232 were significantly decreased (61 significantly decreased). Conclusion: There is significant difference between ESCC and EAC gene expression, these differences may play an important role in different biological behaviors of two kinds of tumors.