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目的:研究化疗药物阿霉素(ADM)联合顺铂(DDP)对宫颈癌CaSki细胞株的增殖及凋亡的影响,并探讨其可能的相互作用机制。方法:应用MTT比色法检测不同浓度阿霉素、顺铂单独和联合应用对宫颈癌CaSki细胞的增殖抑制作用;同时RT-PCR法在mRNA水平上检测Bcl-2和TNF-α基因表达量的变化。结果:两种药物单独应用均可抑制CaSki细胞的增殖,联合用药(<12μg/mL)时具有协同抑制作用并与各药物单一应用比较具有显著性差异(P<0.05);阿霉素、顺铂作用CaSki细胞后能上调TNF-α基因和下调Bcl-2基因的表达。结论:宫颈癌CaSki细胞在化疗药物阿霉素和顺铂两药联合作用下通过诱导TNF-α和Bcl-2 mRNA表达量的变化发挥协同抑制作用,同时TNF-α的高表达增强了化疗药物阿霉素和顺铂诱导肿瘤细胞凋亡的敏感性,其机制主要与凋亡诱导效应有关。
Objective: To study the effects of chemotherapeutic agents adriamycin (ADM) combined with cisplatin (DDP) on the proliferation and apoptosis of cervical cancer cell line CaSki, and to explore its possible interaction mechanism. Methods: MTT assay was used to detect the inhibitory effects of different concentrations of doxorubicin and cisplatin alone or in combination on the proliferation of cervical cancer CaSki cells. Meanwhile, the mRNA expression of Bcl-2 and TNF-α was detected by RT-PCR The change. Results: The two drugs could inhibit the proliferation of CaSki cells alone. Synergistic inhibitory effect was observed when combined with the drug (<12μg / mL) and there was a significant difference (P <0.05) Platinum CaSki cells can up-regulate TNF-α gene and down-regulate Bcl-2 gene expression. CONCLUSIONS: Cervical cancer CaSki cells exert a synergistic inhibitory effect on the expression of TNF-α and Bcl-2 mRNA under the combined action of chemotherapeutic drug doxorubicin and cisplatin. Meanwhile, the high expression of TNF-α enhances the chemotherapeutic drugs The sensitivity of doxorubicin and cisplatin to induce tumor cell apoptosis is mainly related to the apoptosis-inducing effect.