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目的 :观察牛磺酸对钙化的形成及逆转作用的影响 ,探讨牛磺酸在血管钙化发生中的作用。方法 :利用β -甘油磷酸制备钙化血管平滑肌细胞 (VSMCs) ,测定细胞钙含量、碱性磷酸酶活性、[45Ca]沉积及 [3 H] 胸腺嘧啶。结果 :与对照组相比 ,钙化细胞的钙含量、ALP活性和 [45Ca]沉积均明显升高 (P <0 0 5 ) ,而牛磺酸与 β -甘油磷酸同时孵育呈剂量依赖性地抑制钙化发生。在牛磺酸逆转实验中 ,钙化细胞的钙含量、ALP活性和 [45Ca]沉积均较换液前明显降低 (P <0 0 1) ;且牛磺酸呈剂量依赖性地逆转已钙化细胞。与对照组相比 ,钙化细胞的细胞数量和[3 H] TdR掺入量增加 (P <0 0 1) ,牛磺酸早期干预组显著抑制钙化细胞的增殖 ,但牛磺酸对已钙化的细胞 ,增殖抑制效应不明显。结论 :牛磺酸可抑制细胞钙化形成且能逆转已形成钙化。
Objective: To observe the effect of taurine on the formation and reversal of calcification, and to explore the role of taurine in the occurrence of vascular calcification. Methods: Calculated vascular smooth muscle cells (VSMCs) were prepared by using β - glycerophosphate, and the cell calcium content, alkaline phosphatase activity, [45Ca] deposition and [3H] thymidine were measured. Results: Compared with the control group, calcium content, ALP activity and [45Ca] deposition in calcified cells were significantly increased (P <0 05), while taurine and β - glycerophosphate were incubated in a dose - dependent manner Calcification occurs. In the taurine reversal experiment, calcium content, ALP activity and [45Ca] deposition in calcified cells were significantly lower than those before fluid exchange (P <0.01). Taurine reversed the calcified cells in a dose-dependent manner. Compared with the control group, the number of calcified cells and the amount of [3 H] TdR incorporation increased (P <0.01), and the early taurine intervention group significantly inhibited the proliferation of calcified cells. However, Cells, proliferation inhibitory effect is not obvious. Conclusion: Taurine can inhibit the formation of cell calcification and can reverse the formation of calcification.