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探讨 Kupffer细胞在大鼠实验性肝癌细胞凋亡中的作用。应用免疫组化方法和 TUNEL法对单用二乙基亚硝胺(DENA)诱发的肝癌及用氯化钆 (GC)或酵母多糖 (ZM)分别阻塞或激活 Kupffer细胞后 ,给以 DENA所引起的大鼠肝癌中的增殖细胞核抗原 (PCNA)、 bax、 bcl- 2蛋白表达和肝癌细胞的凋亡进行了对比研究。结果显示 :肝癌组织的增殖指数在ZM+DENA组、 DENA组、 GC+DENA组依次增高 ,而凋亡指数在上述各组依次降低。 Bax阳性率在 ZM+DENA组明显高于 DENA组 (P<0 .0 5 ) ,而 ZM+DENA组 bcl- 2阳性率明显低于 DENA组 (P<0 .0 5 )。结果提示 :Kupffer细胞可促进实验性肝癌细胞凋亡。
To investigate the role of Kupffer cells in the apoptosis of rat hepatoma cells. Immunohistochemistry and TUNEL method were used to induce hepatocellular carcinoma induced by diethylnitrosamine (DENA) and blocking or activating Kupffer cells with cesium chloride (GC) or zymosan (ZM), respectively, and then caused by DENA. A comparative study was performed on the expression of proliferating cell nuclear antigen (PCNA), bax, bcl-2 protein and apoptosis of hepatoma cells in rat liver cancer. The results showed that the proliferation index of hepatocellular carcinoma was higher in the ZM+DENA group, the DENA group, and the GC+DENA group, while the apoptotic index was lower in the above groups. The positive rate of Bax was significantly higher in the ZM+DENA group than in the DENA group (P<0.05), while the positive rate of bcl-2 in the ZM+DENA group was significantly lower than that in the DENA group (P<0.05). The results suggest that Kupffer cells can promote the apoptosis of experimental hepatoma cells.