论文部分内容阅读
从两株具有高亲和力及中和活性的抗人肿瘤坏死因子－α（ｈＴＮＦ－α）的单抗杂交瘤细胞株１Ｃ３、３Ｆ６中分别提取总ＲＮＡ，通过ＲＴ－ＰＣＲ扩增并克隆了抗体的ＶＬ、ＶＨ基因。核苷酸序列分析表明，所克隆基因均为抗体的ＶＬ、ＶＨ基因，并分别属于Ｋａｐｐａ链Ⅳ亚组和重链Ⅲ（Ｄ）亚组。此外，两抗体可变区分子模型及与ｈＴＮＦ－α的结合模型的建立及分析，为进一步的基因工程抗体研究及抗原－抗体相互作用机理的研究奠定了基础。
Total RNA was extracted from two McAbs hybridoma cell lines 1C3 and 3F6 with high affinity and neutralizing activity against human tumor necrosis factor-α (hTNF-α) respectively. The total RNA was amplified by RT-PCR and cloned VL, VH gene. Nucleotide sequence analysis showed that all of the cloned genes were the VL and VH genes of the antibody and belong to the subgroup of Kappa chain Ⅳ and the heavy chain Ⅲ (D) respectively. In addition, the construction and analysis of the two antibody variable region molecular models and the binding model with hTNF-α laid the foundation for the further research on the engineered antibody and the research on the antigen-antibody interaction mechanism.