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目的:观察青春前期大鼠睾丸单侧扭转复位后对健侧睾丸的远期影响,并探究生脉注射液对其的保护作用。方法:5周龄健康SD雄性大鼠24只,随机分为生脉注射液组(实验组),生理盐水组(对照组)和假手术组,每组8只,建立左侧睾丸扭转复位模型,术后7周取健侧睾丸及附睾,分别测定睾丸质量和附睾尾中精子活率,睾丸组织内超氧化物歧化酶(SOD),一氧化氮合酶(NOS)活性和丙二醛(MDA)含量。结果:与对照组比较,实验组和假手术组健侧睾丸质量与精子活率,睾丸组织中SOD活性显著升高(P<0.05),NOS活性和MDA含量显著下降(P<0.05)。实验组与假手术组比较,健侧睾丸精子活率与睾丸质量下降,健侧睾丸组织中SOD活性降低,NOS活性和MDA含量升高,但均无统计学意义。结论:青春前期大鼠单侧睾丸扭转复位后可致健侧睾丸损伤,生脉注射液对青春前期大鼠睾丸扭转复位后健侧睾丸损伤远期效果具有一定的保护作用。
OBJECTIVE: To observe the long-term effect of unilateral testicular telangiectasia on contralateral testis in rats and explore the protective effect of Shengmai injection. Methods: Twenty-four healthy SD male rats of 5 weeks old were randomly divided into Shengmai injection group (experimental group), normal saline group (control group) and sham operation group (n = 8) The contralateral testes and epididymis were obtained at 7th week after operation. The testis weight, epididymal sperm motility, the activities of superoxide dismutase (SOD), nitric oxide synthase (NOS) and malondialdehyde (MDA) MDA) content. Results: Compared with the control group, the contralateral testicular weight and sperm motility, the activity of SOD in testis and the activity of NOS and the content of MDA in test group and sham operation group were significantly decreased (P <0.05). Compared with the sham-operation group, the testicular sperm motility and testicular weight decreased, while the SOD activity and the NOS activity and MDA content in the contralateral testicular tissue decreased in the test group and the sham-operated group. However, there was no statistical significance. CONCLUSION: Contralateral testis can be induced by unilateral testicular torsion and reduction in rats, and Shengmai injection has a protective effect on the long-term effect of contralateral testicular damage after pre-admission testicular torsion and reduction.