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目的:探讨磁共振影像技术评价大鼠肌肉组织坏死模型的实验方法,并用病理金标准验证磁共振扫描结果。方法:向6只大鼠左后肢注射0.2 ml无水乙醇,制成骨骼肌坏死模型,分别于造模后0、2、4、8、12、14、16 h进行磁共振扫描,分析图像并得出最佳扫描序列及最佳扫描时间。另取20只模型鼠在最佳扫描条件下行磁共振扫描,扫描后处死行病理检查。对病变区伊文氏蓝染色大体病理图像及T2WI fs图像进行形态比较,并对两者面积比进行分析。结果:T2WI fs时病变范围清晰且排除周围脂肪信号的干扰,为最佳监测序列,12 h为最佳扫描时间。坏死骨骼肌面积在磁共振影像和伊文氏蓝染色大体病理影像上无明显差异。磁共振影像上左后肢坏死骨骼肌面积占可视面积的(7.95±0.18)%,伊文氏蓝染色大体病理影像上左后肢坏死骨骼肌面积占可视面积的(7.91±0.15)%。结论:磁共振影像可作为大鼠骨骼肌坏死模型的评价方法。
Objective: To investigate the experimental method of magnetic resonance imaging (MRI) to evaluate the model of rat muscular tissue necrosis and to verify the results of magnetic resonance scanning with the gold standard of pathology. Methods: The left hind limbs of 6 rats were injected with 0.2 ml of absolute ethanol to make the model of skeletal muscle necrosis. Magnetic resonance imaging was performed at 0, 2, 4, 8, 12, 14 and 16 h after modeling Get the best scan sequence and the best scan time. Another 20 rats were scanned under the best conditions of magnetic resonance scan, the pathological examination was performed after the scan. Morphological comparison of the pathological Evans blue staining and T2WI fs images were performed, and the ratio of the two was analyzed. Results: The T2WI fs had a clear range of lesions and the interference of the surrounding fat signal was eliminated, which was the best monitoring sequence and the optimal scanning time was 12 h. Necrosis skeletal muscle area in magnetic resonance imaging and Evans blue staining of the gross pathological image was no significant difference. The left hindlimb skeletal muscle area accounted for (7.95 ± 0.18)% of the visual field in MR images, and the posterior hind limb necrosis skeletal muscle area accounted for 7.91 ± 0.15% of the visual area in the Evans blue staining gross pathological images. Conclusion: Magnetic resonance imaging can be used as a method to evaluate the skeletal muscle necrosis in rats.