目的:研究莲房原花青素(LSPC)的相对分子量分布及体外抗肿瘤作用。方法:用ESI-MS分析LSPC,MTT法检测人肺癌LETP-2、胃腺癌SGC-7901、黑色素瘤A3753种细胞增殖,采用平板集落实验测定3种癌细胞集落形成。结果:LSPC的分子量范围为290.1～1154.3,是以二聚体为主的低聚原花青素;一定浓度的LSPC对3种瘤细胞均有抑制作用,当LSPC浓度为100μg/mL作用72h时对3种瘤细胞生长抑制率分别为62.6%、49.8%、77.0%;集落形成抑制率分别为77.0%、43.2%、95.3%。结论:LSPC体外对LETP-2、SGC-7901、A3753种瘤细胞有显著的抑制作用。 Objective: To study the relative molecular weight distribution of LSPC and its anti-tumor effect in vitro. METHODS: LSPC was analyzed by ESI-MS. MTT assay was used to detect the proliferation of human lung cancer LETP-2, gastric adenocarcinoma SGC-7901 and melanoma A3753 cells. Plate colony assay was used to determine the colony formation of three kinds of cancer cells. RESULTS: The molecular weight of LSPC ranged from 290.1 ​​to 1154.3, which was a dimer-based oligomeric procyanidins. A certain concentration of LSPC inhibited all three kinds of tumor cells. When the concentration of LSPC was 100 μg/mL for 72 h, there were three kinds of LSPC. The tumor cell growth inhibition rates were 62.6%, 49.8%, and 77.0%, respectively; the inhibition of colony formation was 77.0%, 43.2%, and 95.3%, respectively. Conclusion: LSPC has significant inhibitory effect on LETP-2, SGC-7901 and A3753 tumor cells in vitro.