目的：检测血管内皮细胞蛋白酪氨酸磷酸化水平对内皮屏障功能的影响。方法：利用荧光比率测定技术测定猪游离冠状微静脉的通透性。利用Ｗｅｓｔｅｒｎ印迹法和重组抗磷酸酪氨酸多克隆抗体测定内皮细胞蛋白酪氨酸磷酸化水平。结果：磷酸酪氨酸水解酶抑制剂苯砷化氧（ＰＡＯ，浓度为０．１、１、１０和１００μｍｏｌ／Ｌ）可呈剂量依赖性地增加微静脉的通透性，分别为给药前的１４９．４２％±９．７１％、１６６．３６％±６．３８％、１９５．２０％±１５．３０％和２１９．２８％±２４．１２％。酪氨酸激酶抑制剂ｄａｍｎａｃａｎｔｈａｌ则可明显减弱组胺或佛波酯醇（ＰＭＡ）介导的血管高通透性反应。ＰＡＯ和组胺及ＰＭＡ都可明显增加培养的内皮细胞骨架蛋白（６５ｋｄ）的酪氨酸磷酸化水平。结论：蛋白酪氨酸磷酸化在激动剂介导的微血管高通透性反应中起着重要作用 Objective: To detect the effect of protein tyrosine phosphorylation of vascular endothelial cells on endothelial barrier function. Methods: Fluorescence ratio was used to measure the permeability of porcine isolated coronary venules. Western blotting and recombinant anti-phosphotyrosine polyclonal antibody were used to determine the protein tyrosine phosphorylation of endothelial cells. RESULTS: Phosphotyrosine hydrolase inhibitor benzene arsenic oxide (PAO, at concentrations of 0.1, 1, 10 and 100 μmol / L) increased the permeability of venules in a dose-dependent manner, 149.42% ± 9.71%, 166.36% ± 6.38%, 195.20% ± 15.30% and 219.28% ± 24.12%, respectively. The tyrosine kinase inhibitor damnacanthal significantly attenuated histamine or phorbol ester (PMA) -mediated vascular hyperpermeability. Both PAO and histamine and PMA significantly increased tyrosine phosphorylation of cultured endothelial cytoskeletal protein (65 kd). CONCLUSION: Protein tyrosine phosphorylation plays an important role in agonist-mediated microvascular hyperpermeability