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用 ＰＣＲ法扩增并克隆了 ｐｒｏｆｉｌｉｎ２全长启动子（１６６７ ｂｐ），经 ５’端不同长度缺失，与 ｇｕｓ（ｕｉｄＡ）基因连接，构建植物表达载体，转化伽蓝菜，证实在转基因植株中全长启动子Ｐｆｎ１．７呈维管束特异表达．５’端缺失分析显示，可将该启动子分为 ３个区段：区段 １，－１６６７—１３８０ tｂｐ，缺失该区段后 ｇｕｓ基因由维管束特异表达变为组成型表达，推测在该区段中存在维管束特异表达元件；区段 ２，－１１５３～－５９７ ｂｐ，强烈抑制 ｇｕｓ基因的表达；区段 ３，－５９７～－１ｂｐ，可认为是ｐｒｏｆｉｌｉｎ２的基本启动子．
The full-length promoter of profilin2 (1667 bp) was amplified by PCR and ligated with gus (uidA) gene with different length at the 5 ’end to construct plant expression vector. Promoter Pfn1.7 showed vascular bundle-specific expression. The 5 ’deletion analysis showed that the promoter could be divided into 3 segments: segment 1, -1667-1380 tbp. After deletion of this segment, gus gene was changed from vascular bundle-specific expression to constitutive expression. In this region, vascular bundle specific expression elements were present; segment 2, -1153 ~ -597 bp, strongly inhibited the expression of gus gene; segment 3, -597 ~ -1bp, could be considered as the basic promoter of profilin2.