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目的:研究不同浓度纤维蛋白原(Fg)、纤维蛋白(Fb)及其降解产物(FDPs)对人脐静脉内皮细胞(HUVECs)/平滑肌细胞(SMCs)共培养模型中HUVECs趋化和迁移的影响。方法:以Transwell膜为载体,建立原代培养的HUVECs与兔SMCs共培养体系,应用不同浓度的Fg、Fb和FDPs干预共培养体系,观察HUVECs趋化(通过Transwell膜装置)和迁移(通过细胞刮伤模型)情况。结果:共培养的HUVECs和SMCs在Transwell膜上生长良好,膜两侧的HUVECs和SMCs可以发生细胞连接。Fg≥3.0 mg·mL-1呈浓度依赖性地促使HUVECs的趋化和迁移。Fb≥1.5 mg·mL-1时呈浓度依赖性地促使HUVECs的趋化和迁移数增加(P<0.05)。FDPs≥0.5 mg·mL-1时HUVECs的趋化和迁移数明显增加(P<0.05)。结论:利用Transwell膜建立的HUVECs/SMCs共培养体系能较好地模拟血管壁的结构关系,为进一步开展相关研究奠定了细胞模型的基础。一定浓度的Fg、Fb及其FDPs可以促进HUVECs趋化与迁移过程,在动脉粥样硬化的发生发展中起重要作用。
AIM: To investigate the effects of different concentrations of Fg, Fb and FDPs on the chemotaxis and migration of HUVECs in co-cultured human umbilical vein endothelial cells (HUVECs) / smooth muscle cells (SMCs) . Methods: Co-culture system of primary cultured HUVECs and rabbit SMCs was established by using Transwell membrane as a carrier. The co-culture system of HUVECs and rabbit SMCs was established by using different concentrations of Fg, Fb and FDPs. The chemotaxis of HUVECs (via Transwell membrane device) and migration Scratch model) situation. Results: Co-cultured HUVECs and SMCs grew well on Transwell membrane, and cell junctions could occur between HUVECs and SMCs on both sides of the membrane. Fg≥3.0 mg · mL-1 promoted chemotaxis and migration of HUVECs in a concentration-dependent manner. When Fb≥1.5 mg · mL-1, the chemotactic and migration of HUVECs increased in a concentration-dependent manner (P <0.05). The chemotaxis and migration of HUVECs were significantly increased when FDPs≥0.5 mg · mL-1 (P <0.05). CONCLUSION: The HUVECs / SMCs co-culture system established by Transwell membrane can better simulate the structure of vascular wall and lay the foundation of cell model for further research. A certain concentration of Fg, Fb and FDPs can promote the chemotaxis and migration of HUVECs and play an important role in the development of atherosclerosis.