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目的探讨鸟苷酸结合蛋白基因β3亚单位(G-proteinβ3Subunit,GNB3)第10外显子C825T多态同中国哈萨克族人群肥胖的关系。方法应用PCR技术及限制性长度多态(RFLP)技术在277例哈萨克族正常体重者,121例超重者及102例肥胖者中检测GNB3基因C825T多态性。结果GNB3基因C825T多态3种基因型分别为TT、CT及CC。其中CT、TT基因型分布频率在正常体重组、超重组及肥胖组分别为46.9%,54.5%,54.9%,23.4%,16.6%,20.6%,基因型分布在组间无显著性差异(P=0.39)。T等位基因分布在正常体重组、超重组及肥胖组分别为46.9%,43.8%,48.0%,组间也无显著性差异(P=0.63)。将正常体重及超重者按高血压及正常血压分层,发现超重及肥胖的高血压者TT基因型频率(26.2%)较正常体重的高血压者(14.7%)有增高趋势(P=0.061)。结论GNB3基因825T等位基因可能不是中国哈萨克族肥胖的主要遗传易感因子,但TT基因型可能在超重的高血压者中有一定作用,此基因在中国其他人群肥胖合并高血压中的作用有必要进行深入研究。
Objective To investigate the relationship between the C825T polymorphism of exon 10 of G-proteinβ3 subunit (GNB3) and the obesity of Chinese Kazak population. Methods PCR-RFLP technique was used to detect the C825T polymorphism of GNB3 gene in 277 Kazakh normal weight subjects, 121 overweight subjects and 102 obese subjects. Results The genotypes of GNB3 C825T polymorphism were TT, CT and CC respectively. The frequencies of CT and TT genotypes were 46.9%, 54.5%, 54.9%, 23.4%, 16.6% and 20.6% respectively in normal weight group, overweight group and obesity group (P = 0.39). The allele distribution of T allele was 46.9%, 43.8% and 48.0% in normal weight group, overweight group and obesity group respectively. There was no significant difference between the two groups (P = 0.63). Stratified hypertension and normotension according to normal body weight and overweight, and found that the frequency of TT genotype (26.2%) in overweight and obese hypertensive patients was higher than that of normal weight hypertensive patients (14.7%) (P = 0.061) . Conclusion The 825T allele of GNB3 gene may not be the major genetic predisposition to obesity in Kazakhs in China. However, the TT genotype may play a role in overweight hypertensive patients. The role of this gene in obesity and hypertension in other Chinese populations Necessary for in-depth study.