论文部分内容阅读
目的探讨异常胆液质载体溃疡性结肠炎大鼠结肠组织中炎症相关因子白介素(interleukin,IL)-1a、IL-1b、IL-10在异常胆液质载体溃疡性结肠炎病证发生、发展中的作用.方法根据维吾尔医学体液理论建立异常胆液质载体证候模型的基础上,采用2,4,6-三硝基苯磺酸(trinitro-benzene-sulfonic acid,TNBS)/乙醇法构建异常胆液质载体溃疡性结肠炎(ulcerative colitis,UC)病证大鼠模型,并进行鉴定,应用实时荧光定量逆转录聚合酶链反应(quantitative reverse transcriptionpolymerase chain reaction,qRT-PCR)方法 ,检测正常组与异常胆液质载体UC病证模型组(模型组)大鼠结肠组织中I L-1a、I L-1b、IL-10 mRNA表达水平,并分析其表达差异.结果模型鉴定与HE染色结果显示,模型组大鼠体征、症状、结肠黏膜损伤等均符合异常胆液质载体UC病证模型的判定标准;qRT-PCR结果显示,与正常组比较,模型组大鼠结肠组织中IL-1a、IL-1b、IL-10的mRNA表达水平均上调,差异有统计学意义(P<0.05).结论异常胆液质载体UC病证模型组大鼠结肠组织中存在炎症相关因子平衡紊乱.
Objective To investigate the occurrence and development of inflammatory factors interleukin (IL) -1a, IL-1b and IL-10 in colonic tissue of abnormal bile-fluid-carrying ulcerative colitis rats .Methods According to the Uyghur medical theory of body fluid, we established a model of abnormal cholacadic carrier syndrome by using 2,4,6-trinitro-benzene-sulfonic acid (TNBS) / ethanol The model of ulcerative colitis (UC) was established and identified. The normal and normal controls were detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR) The expression of IL-1a, IL-1b and IL-10 mRNA in the colon tissue of rats with abnormal bile-fluid-bearing UC syndrome model (model group) and their differences were analyzed.Results The results of model identification and HE staining The results showed that the model group rats signs, symptoms, colonic mucosal injury and so on are in line with the abnormal choledochiocarcinoma UC disease model to determine the standard; qRT-PCR results showed that compared with the normal group, the model group rats colon IL- 1a, IL-1 (P <0.05) .Conclusion Inflammatory factor-related imbalance exists in the colon tissue of rats with abnormal bile-fluid-bearing UC syndrome model.