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为了挖掘、开发我国枸杞种质资源,本项研究采用超高效液相色谱-高分辨质谱法[UPLC-MS(HRMS)]、滴定分析法、比色法等方法,对宁夏野生苦味枸杞的主要化学成分、苦味物质进行了观察和检测。结果表明:宁夏野生苦味枸杞的多糖、甜菜碱含量分别比栽培枸杞宁杞7号高54.5%~74.2%、26.7%~30.0%。初步判定,苦味物质的主要来源可能为具有二氢槲皮素结构的黄酮类化合物,其中最主要物质的分子量为304.2626。
In order to excavate and develop the germplasm resource of Lycium barbarum in our country, this study mainly used the method of ultra-high performance liquid chromatography-high resolution mass spectrometry [UPLC-MS (HRMS)], titration analysis and colorimetric method Chemical composition, bitter substances were observed and detected. The results showed that the contents of polysaccharides and betaines in Ningxia wild bitter Chinese wolfberry were 54.5% -74.2% and 26.7% -30.0% higher than that of Ningxia 7. It was initially determined that the main source of bitter substances may be flavonoids with dihydroquercetin structure, of which the most important substance has a molecular weight of 304.2626.