目的研究聚偏氟乙烯(PVDF)膜分离地龙匀浆液最适宜的孔径。方法以新鲜红地龙的匀浆液为分离对象,采用不同孔径的聚偏氟乙烯膜进行超滤,对超滤液进行蛋白浓度和蛋白分子量分布的测定。结果截留分子量(MWCO)为50000的膜对地龙匀浆液的超滤结果最好,其截留液蛋白浓度大,分子量分布在1.5~9U之间。结论用聚偏氟乙烯膜分离地龙匀浆液,这种常温下进行、不需添加其他化学试剂且操作时间短的方法对制备较高浓度地龙蛋白液是切实可行的。 Objective To study the optimum pore size of polyvinylidene fluoride (PVDF) membrane for separation of groundwater homogenate. Methods The homogenate of fresh red earth dragon was used as the object of separation. Ultrafiltration was performed using polyvinylidene fluoride membranes with different pore sizes. The ultrafiltrate was measured for protein concentration and protein molecular weight distribution. Results The membrane with MWCO of 50,000 had the best ultrafiltration results for the homogenate. The concentration of protein in the retentate was large and the molecular weight distribution was between 1.5 and 9 U. Conclusion Separation of groundwater homogenate with polyvinylidene fluoride membrane, which is carried out at room temperature without adding other chemical reagents and with a short operating time, is feasible for the preparation of higher concentrations of earthworm protein liquid.