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目的:建立微波消解石墨炉原子吸收光谱法(GFAAS)测定大鼠组织中的微量硒的检测方法。方法:采用微波消解法处理样品,用硝酸作为消解剂进行微波消解;采用氯化钯则作为基体改进剂来测定样品中的微量硒。结果:最佳灰化温度为900℃,原子化温度为2500℃;脑组织和肺组织的相对标准偏差(RSD)分别为2.83%和3.05%,样品加标回收率为97%~105%。结论:该方法用于检测大鼠组织中的硒含量,操作简便、快速、准确度好、结果满意,能够适用于生物体内多种组织器官中硒含量的测定与分析。
Objective: To establish a method for the determination of trace selenium in rat tissues by microwave digestion and graphite furnace atomic absorption spectrometry (GFAAS). Methods: The samples were treated by microwave digestion and nitric acid was used as the digestion reagent for microwave digestion. Palladium chloride was used as matrix modifier to determine trace selenium in the samples. Results: The best ashing temperature was 900 ℃ and the atomization temperature was 2500 ℃. The relative standard deviations (RSDs) of brain tissue and lung tissue were 2.83% and 3.05%, respectively. The spiked recoveries were 97% -105%. Conclusion: This method is suitable for the determination of selenium content in rat tissues. It is simple, rapid and accurate. The method is satisfactory and can be applied to the determination and analysis of selenium content in various tissues and organs of organism.