论文部分内容阅读
[Objective] To establish a method to determine the content of bergenin in RHIZOMA BERGENIAE SCOPULOSAE by HPLC.[Method] The research was carried out on Kromasil-C18 column(4.6 mm×150 mm,5 μm) .And the mobile phase was acetonitrile-0.2% phosphoric acid(10∶90) with a flow rate of 1.0 ml/min.The column temperature was 35 ℃ and the detection wavelength was 275 nm.Thus,the content of bergenin in RHIZOMA BERGENIAE SCOPULOSAE was detected.[Result] Bergenin showed good linear relationship between the range of 0.248 and 1.240 μg/ml(r=0.997 9) ;and the average recovery was 100.95% with RSD=2.47%(n=6) .[Conclusion] This method was simple,repeatable and accurate and could be used for the quality control of RHIZOMA BERGENIAE SCOPULOSAE.
[Objective] To establish a method to determine the content of bergenin in RHIZOMA BERGENIAE SCOPULOSAE by HPLC. [Method] The research was carried out on Kromasil-C18 column (4.6 mm × 150 mm, 5 μm) .And the mobile phase was acetonitrile The content of bergenin in RHIZOMA BERGENIAE SCOPULOSAE was detected. [Result] Bergenin showed good linear relationship between the range of 0.248 and 1.240 μg / ml (r = 0.997 9); and the average recovery was 100.95% with RSD = 2.47% (n = 6). [Conclusion] This method was simple, repeatable and accurate and could be used for the quality control of RHIZOMA BERGENIAE SCOPULOSAE.