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目的 探讨肾脏疾病患儿可溶性白细胞介素 2受体 (sIL 2R)和T细胞亚群的改变及其临床意义。方法 用ELISA法检测 62例肾病综合征、48例肾小球肾炎患儿及 3 0例健康对照儿童血清和尿中sIL 2R浓度 ,单克隆抗体间接免疫荧光法检测T细胞亚群。结果 (1)肾病综合征及肾小球肾炎活动期组血清及尿sIL 2R浓度分别高于各自缓解期组 (P <0 .0 5 )和对照组 (P <0 .0 5 ) ;各自缓解期组血清和尿sIL 2R浓度与对照组间差异无显著意义 (P >0 .0 5 )。 (2 )肾病综合征和肾小球肾炎活动期患儿尿与血清中sIL 2R浓度呈正相关 (P <0 .0 1)。 (3 )肾病综合征活动期组CD4 + 及CD4 + /CD8+ 明显低于该病缓解期组和对照组 (均P <0 .0 1)。结论 肾病综合征和肾小球肾炎活动期患儿T细胞免疫应答能力低下 ,sIL 2R升高可作为诊断肾脏疾病是否活动的有用指标
Objective To investigate the changes of soluble interleukin 2 receptor (sIL 2R) and T lymphocyte subsets in children with renal disease and its clinical significance. Methods Serum and urinary sIL 2R concentrations were measured by ELISA in 62 cases of nephrotic syndrome, 48 cases of glomerulonephritis and 30 healthy controls. T cell subsets were detected by indirect immunofluorescence assay. Results (1) Serum and urinary sIL 2R levels in nephrotic syndrome and glomerulonephritis active stage were higher than those in remission stage (P <0.05) and control group (P0.05) Serum and urine sIL 2R levels in the control group were not significantly different between the two groups (P> 0.05). (2) There was a positive correlation between urine and serum sIL 2R concentration in patients with nephrotic syndrome and glomerulonephritis (P <0.01). (3) CD4 + and CD4 + / CD8 + in active stage of nephrotic syndrome were significantly lower than those in remission stage and control group (all P <0.01). Conclusion Nephrotic syndrome and glomerulonephritis in children with active immune response to low T cell immune response, elevated sIL 2R can be used as a useful indicator of diagnosis of renal disease activity