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目的探讨自发性高血压大鼠(SHR)蛋白质组学的发病机制。方法以SHR10只为模型组,Wistar Kyoto大鼠10只为正常组,取主动脉血,低温离心,分离血清,每组随机各取5份血清样品,用双向凝胶电泳、基质辅助激光解析电离飞行时间质谱测定肽质量指纹图谱,数据库检索鉴定各肽段对应的蛋白质,获取血清差异表达蛋白质的信息。结果 2组均获得重复性好的血清蛋白质双向凝胶电泳图谱。模型组和正常组凝胶上的平均蛋白点分别为(527±34)个、(476±30)个。通过分析蛋白质点局部图谱,获得23个差异表达蛋白点。与正常组比较,模型组4个点高表达,17个点低表达,2个点缺失。23个差异点经鉴定可以确认的蛋白质点有:线粒体膜蛋白、锌指蛋白、Rho GTPase、T细胞受体、视黄醇结合蛋白、假想蛋白。结论线粒体膜蛋白、锌指蛋白、T细胞受体、假想蛋白在SHR低表达;Rho GTPase、视黄醇结合蛋白在SHR高表达,推测这些蛋白可能与高血压的发生、发展密切相关。
Objective To investigate the pathogenesis of proteomics in spontaneously hypertensive rats (SHR). Methods SHR10 was used as a model group and 10 Wistar Kyoto rats as a normal group. Serum was collected from the aorta and centrifuged at low temperature. Five serum samples were obtained from each group randomly. Two - dimensional gel electrophoresis, matrix - assisted laser desorption ionization Time of flight mass spectrometry peptide mass fingerprinting, database search Identify the corresponding protein of each peptide to obtain serum differentially expressed protein information. Results Both groups had good reproducibility of serum protein two-dimensional gel electrophoresis. The average protein spots on the gel of model group and normal group were (527 ± 34) and (476 ± 30), respectively. By analyzing the protein spots map, 23 differentially expressed protein spots were obtained. Compared with the normal group, the model group 4 points high expression, 17 points low expression, 2 points missing. The 23 spots identified identified proteins as mitochondrial membrane proteins, zinc finger proteins, Rho GTPases, T cell receptors, retinol binding proteins, hypothetical proteins. Conclusions Mitochondrial membrane protein, zinc finger protein, T cell receptor and hypothetical protein are low expressed in SHR. Rho GTPase and retinol binding protein are highly expressed in SHR, suggesting that these proteins may be closely related to the occurrence and development of hypertension.