本文采用正交试验设计优选ConA 诱导的人外周血淋巴细胞化学发光(PBL—CL)测定体系中PBL、Luminol 及ConA 等三个主要因素的最佳组合。结果表明,PBL—CL 测定的最优实验条件为:0.5ml PBL 悬液(1×10~6/ml)、0.2ml Luminol 溶液(5×10~(-4)M)及0.2ml ConA 溶液(500μg/ml),反应液总量为1.0m。PBL—CL 测定是一种简便、怏速、敏感的检测淋巴细胞早期活化及功能的新方法。 In this paper, the best combination of the three major factors of PBL, Luminol and ConA in ConA-induced human peripheral blood lymphocyte chemoluminescence (PBL-CL) system was optimized by orthogonal design. The results showed that the optimum conditions for the determination of PBL-CL were as follows: 0.5ml PBL suspension (1 × 10 ~ 6 / ml), 0.2ml Luminol solution (5 × 10 ~ (-4) M) and 0.2ml ConA solution 500 μg / ml), and the total amount of the reaction solution was 1.0 m. PBL-CL assay is a simple, rapid and sensitive method to detect early activation and function of lymphocytes.