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目的:比较开放式、封闭式玻璃化冷冻技术及程序化慢速冷冻法对辅助生殖技术人早期胚胎冷冻复苏的效果。方法:回顾性分析622例胚胎冷冻复苏资料,将体外受精-胚胎移植(IVF-ET)患者第3天需要冷冻的胚胎,分别采用开放式、封闭式麦管玻璃化法及程序化慢速冷冻法冷冻,比较3组胚胎复苏率、空泡出现率、胚胎完整率、植入率、临床妊娠率、周期取消率、胚胎丢失率及流产率。结果:开放式冻存组共复苏280个周期、627个胚胎,胚胎复苏率、胚胎完整率、植入率和临床妊娠率分别为97.13%、94.89%、28.90%、52.61%,与程序化冷冻组(复苏165个周期、477个胚胎)胚胎复苏率(73.79%)、胚胎完整率(70.02%)、植入率(16.75%)和临床妊娠率(35.44%)相比差异有统计学意义(P<0.05),与封闭式冻存组(复苏177个周期、400个胚胎)相比差异均无统计学意义(P>0.05),3种冷冻组空泡出现率、周期取消率、胚胎丢失率及流产率差异均无统计学意义(P>0.05)。结论:两种玻璃化冷冻法能获得同样的冷冻效率,且均优于程序化冷冻法;封闭式麦管玻璃化法能更好地保存胚胎复苏后的发育潜能,是冻存人早期胚胎较为理想的方法。
OBJECTIVE: To compare the effects of open and closed vitrification and programmed cryosurgery on cryopreservation of early embryos in assisted reproductive technology. Methods: The data of 622 frozen embryos were retrospectively analyzed. The frozen embryos on the third day after in vitro fertilization-embryo transfer (IVF-ET) were treated with open, closed-tube method and programmed freezing Frozen, the embryo recovery rate, the incidence of vacuoles, embryo intact rate, implantation rate, clinical pregnancy rate, cycle cancellation rate, embryo loss rate and abortion rate were compared. Results: In the open cryosurgery group, a total of 280 cycles were recovered. 627 embryos, embryo recovery rate, embryo intact rate, implantation rate and clinical pregnancy rate were 97.13%, 94.89%, 28.90% and 52.61% There were significant differences in embryo recovery rate (73.79%), embryo complete rate (70.02%), implantation rate (16.75%) and clinical pregnancy rate (35.44% (P <0.05). There was no significant difference between the frozen-thawed group and the frozen-thawed group (177 cycles of resuscitation and 400 embryos) (P> 0.05). The incidences of vacuoles, Rate and abortion rate differences were not statistically significant (P> 0.05). CONCLUSION: Both vitrification methods can achieve the same freezing efficiency, both of which are better than programmed freezing method. The closed tube vitrification method can better preserve the embryonic development potential after cryopreservation, The ideal way.