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本文用抗人肝癌细胞的单克隆抗体,采用ABC法,50例临床病理诊断为肝细胞肝癌及正常组织等的石蜡切片进行了免疫组化研究,发现该抗体染色在肝癌阳性率为88％(44/50例),而在全身绝大部份脏器的正常组织无交叉反应。这一结果表明,该抗体对肝癌组织具有一定的特异性。为证实此抗体定位的相应抗原部位,我们还通过免疫电镜包埋前和包埋后两种不同的染色方法,对5例临床手术切除的新鲜肝癌标本进行了超微结构定位观察。结果,此抗体主要定位于细胞膜性结构,包括细胞膜连接,线粒体膜和内质网膜。推测此抗体为肝癌细胞相关膜抗原的单克隆抗体。此抗原物质的发现,为肝癌诊断及研究展示了新的前景。
In this article, immunohistochemical studies using paraffin sections of anti-human hepatoma cells using ABC method and 50 cases of clinical pathological diagnosis of hepatocellular carcinoma and normal tissues, found that the antibody staining in liver cancer positive rate was 88% ( 44/50 cases), but there is no cross reaction in the normal tissues of most organs throughout the body. This result shows that the antibody has specificity for hepatocarcinoma tissue. In order to confirm the location of the corresponding antigen in this antibody localization, we also performed ultrastructural localization observations on 5 cases of freshly resected liver cancer specimens by two different staining methods before and after embedding by immunoelectron microscopy. As a result, this antibody is mainly located in the cell membrane structure, including cell membrane junction, mitochondrial membrane and endoplasmic reticulum membrane. It is speculated that this antibody is a monoclonal antibody to a membrane-associated antigen of liver cancer cells. The discovery of this antigen has shown new prospects for the diagnosis and research of liver cancer.