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植物名称:桑(Morus)品种为火桑、剑持等(Morus bombycis)。材料类别:20年生植株的冬芽芽芯。早春桑芽萌发前,剪取一年生枝条的中、上部分,以塑料薄膜包裹后贮藏于3—4℃温度下经25—45天。接种前将枝条洗净并截成约1.5cm的小段,每段含一完整芽,经常规方法灭菌后于无菌条件下用解剖刀剥去芽鳞及外层叶片,切取长3—4mm的芽芯作培养材料。部养条件:以改动的 MS为基本培养基,每升附加细胞分裂素BA0.5mg,生长素IAA1.0mg培养基的蔗糖(食用白糖)和琼脂浓度分别为3％和
Botanical Name: Morus Varieties are Morus bombycis. Material Type: Buds of winter buds of 20-year-old plants. Early spring mulberry germination before clipping the annual branches in the upper part of the plastic film wrapped stored at 3-4 ℃ after 25-45 days. Before inoculation, the branches were washed and cut into small pieces of about 1.5cm, each containing a complete bud, sterilized by conventional methods under aseptic conditions with a scalpel stripped bud scales and outer leaves, cut length 3-4mm Bud core for culture material. Parting conditions: the modified MS as the basic medium, added per liter of cytokinin BA0.5mg, auxin IAA1.0mg medium sucrose (edible sugar) and agar concentrations were 3% and