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目的探讨格列卫(gleevec)对耐药卵巢癌细胞COC1增殖、顺铂敏感性和血管形成的影响,为临床应用格列卫治疗难治性卵巢癌提供实验依据。方法2006年9月至2007年5月于哈尔滨医科大学第一临床医学院采用四甲基偶氮唑蓝(MTT)法检测格列卫对细胞增殖的影响;采用ELISA法检测格列卫对COC1表达血管内皮细胞生长因子(VEGF)和内皮抑素(endostatin)的影响。结果格列卫对COC1细胞增殖的抑制作用表现出浓度依赖性,并显著提高了其对顺铂的敏感性;格列卫对COC1表达VEGF具有明显的抑制作用,格列卫对COC1表达内皮抑素具有明显促进作用,并呈浓度依赖性。结论格列卫能够抑制顺铂耐药卵巢癌细胞COC1的增殖,并显著增强其对顺铂的敏感性;格列卫对抑制肿瘤细胞产生VEGF具有明显的作用。
Objective To investigate the effects of gleevec on the proliferation, cisplatin sensitivity and angiogenesis of drug-resistant ovarian cancer cell line COC1 and to provide experimental evidence for the treatment of refractory ovarian cancer with Gleevec. Methods From September 2006 to May 2007, MTT assay was used to detect the effect of Glivec on cell proliferation in the First Clinical College of Harbin Medical University. The effects of Glivec on the expression of COC1 Expression of vascular endothelial growth factor (VEGF) and endostatin. Results Glivec showed a concentration-dependent inhibition of COC1 cell proliferation and significantly increased its sensitivity to cisplatin. Glivec showed a significant inhibitory effect on the expression of COC1 in VEGF. Glivec inhibited the expression of COC1 in endothelial cells Su has a significant role in promoting, and in a concentration-dependent manner. Conclusion Gleevec can inhibit the proliferation of cisplatin-resistant ovarian cancer cells COC1 and significantly enhance its sensitivity to cisplatin. Glivec has a significant effect on the inhibition of VEGF production by tumor cells.