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本文研究建立一套高效、简便的草地土壤微生物DNA的提取和纯化方法.提取的微生物总DNA片段大小在23 kb左右,纯化后的DNA产量为(7.76±1.53)μg·g-1,OD260/OD280为1.78±0.04,OD260/OD230为1.76±0.02.“,”The aim of the study was to establish a highly efficient,simple method to extract and purify soil microbial DNA.The extraction of microbial DNA fragment was about 23 kb in size,the yield of DNA solution after purification was(7.76 ± 1.53)μg.g-1,OD260/OD280 was 1.78 ± 0.04,OD260/OD230 was 1.76 ± 0.02.