Objective To explore the effect of p27 gene on the growth inhibition of laryngeal carcinoma cell line Hep 2. Methods The p27 cDNA was transfected into human laryngeal carcinoma cell line Hep 2 cells with lipofectamine. The cell cycles were observed by means of FCM assay. p27 expression was detected by dot blot hybridization and Western blot. Results Expression of p27 in Hep 2 was identified by Dot blot and Western blot analyses.the growth rate of Hep 2 transfected with p27 gene was markedly suppressed. Cell cycle analysis by flow cytometry show that the number of cells in G0～ G1 phase of Hep 2 cells was significantly increased while cells in S and G2+ M phase was decreased compared with that of the control Hep 2 cells. Conclusion Transduction of p27 gene into lower expression cancer cells can restore its suppressive effect on cell growth by arrest of cell cycle at G1 phase.