应用细胞培养技术对新生牛主动脉平滑肌细胞进行传代培养,取第4～6代平滑肌细胞作为受试对象研究不同浓度胰岛素对牛生动脉平论肌细胞内脂质含量的影响,采用Heider改良法（即酶-荧光法）测定平滑肌细胞内微量胆固醇。结果显示:胰岛素增加平滑肌细胞内胆固醇的含量,且胰岛素浓度与平滑肌细胞内胆固醇含量是正相关。上述结果提示:胰岛素可促进平滑肌细胞内脂质蓄积,从另一侧面加速动脉粥样硬化的发生与发展。 The cell culture technique was used to subculture newborn bovine aortic smooth muscle cells. The 4th to 6th generation smooth muscle cells were used as subjects to study the effect of different concentrations of insulin on lipid content in bovine arterial smooth muscle cells. Heider modified method (Ie, enzyme - fluorescence method) Determination of trace cholesterol in smooth muscle cells. The results showed that insulin increased the content of cholesterol in smooth muscle cells, and insulin concentration was positively correlated with the content of cholesterol in smooth muscle cells. The above results suggest that: Insulin can promote lipid accumulation in smooth muscle cells, accelerate the occurrence and development of atherosclerosis from the other side.