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目的 建立子宫内膜异位症 (内异症 )裸鼠模型 ,并对其相关生物学行为进行研究。方法 取人分泌晚期子宫内膜 ,剪碎 ,置入裸鼠盆、腹腔不同部位 ,内异症 (EM组 )和非内异症 (NEM组 )裸鼠各 2 4只 ;对照组 :3只裸鼠 ,同法将人大网膜组织置入裸鼠盆、腹腔不同部位。各组裸鼠分别于置入后第 5、15、30天随机脱颈椎处死 ,观察异位病灶生长情况 ,并留取异位病灶 ,在光镜及透射电镜下观察其形态学变化。RT PCR法检测在位内膜及异位病灶血管内皮生长因子 (VEGF)、基质金属蛋白酶 9(MMP 9)mRNA表达 ,免疫组化链霉素抗生物素蛋白 过氧化物酶连接法检测异位病灶雌、孕激素受体表达。结果 内膜置入 5d ,即见异位病灶牢固黏附 ,血液供应丰富。光镜下见腺体细胞及散在间质细胞 ,且随时间延长 ,异位病灶与裸鼠组织融合越明显。 15d时 ,电镜下可见异位病灶内炎性细胞增生、浸润最明显 ,30d时腺细胞中细胞器消失 ,但仍可见分泌颗粒 ,核染色质聚集 ,并有凋亡趋势。与在位内膜比较 ,异位病灶VEGF、MMP 9mRNA表达增强 (P <0 0 5 ) ,异位内膜置入第 15天时表达最强 (VEGF :EM组为 1 11± 0 13、NEM组为 1 10± 0 11;MMP 9:EM组为 1 0 0± 0 11、NEM组为 0 99± 0 0 8) ,第 30天时有所下降 (VEGF :EM组为 0 85± 0 1
Objective To establish a nude mouse model of endometriosis (endometriosis) and to study its related biological behavior. Methods The human endometrium was secreted by endometriosis, cut into pieces, placed in nude mice ’s stomach, different parts of abdominal cavity, endometriosis (EM group) and non - endometriosis (NEM group) nude mice respectively. Control group: 3 Nude mice, with the law of human omentum into nude mice pots, different parts of the abdominal cavity. Nude mice in each group were sacrificed on the 5th, 15th and 30th days after the operation. The ectopic lesions were observed and the ectopic lesions were collected. Morphological changes were observed under light and transmission electron microscopy. RT-PCR was used to detect the expression of vascular endothelial growth factor (VEGF) and matrix metalloproteinase 9 (MMP 9) mRNA in eutopic and ectopic lesions, and immunohistochemical streptavidin-avidin-peroxidase Focal estrogen and progesterone receptor expression. Results endometrial put 5d, that ectopic lesions solid adhesion, rich in blood supply. Light microscope, see glandular cells and scattered interstitial cells, and with time, ectopic lesions and nude mice tissue fusion more obvious. On the 15th day, the inflammatory cells proliferated and infiltrated most obviously in the ectopic lesion under the electron microscope, and the organelles disappeared in the gland cells on the 30th day. However, the secretory granules and nuclear chromatin were still visible and showed the tendency of apoptosis. Compared with the eutopic endometrium, the expression of VEGF and MMP 9 mRNA in ectopic lesions increased (P <0.05), and the ectopic endometrium had the strongest expression on the 15th day (VEGF: 1 11 ± 0 13 in EM group, NEM group 1 10 ± 0 11; MMP 9: 1 0 0 ± 0 11 in the EM group and 0 99 ± 0 0 8 in the NEM group), and decreased on the 30th day (VEGF: 0 85 ± 0 1 in the EM group