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目的 :探讨含硒制剂诱导金属硫蛋白在对抗阿霉素心脏毒性中的作用 ,比较无机硒制剂亚硒酸钠与有机硒制剂富硒麦芽。方法 :不同时间给小鼠喂予硒制剂后取血分离血清 ,按常规查GOT和CK活性 ,按DTNB法测GSH Px活性 ;取心、肝、肾组织按Hg Chelex法测金属硫蛋白 (MT)含量并做心脏病理学检查。结果 :两种含硒制剂均能减轻阿霉素引起的体重下降、GOT与CK酶升高及心脏病理损害 ,其中富硒麦芽的效果略优于亚硒酸钠。两者还能增加GSH Px酶活性 ,诱导心脏中MT合成增加 ,连续使用硒制剂的整体效果优于先或后使用该制剂。结论 :除升高GSH Px外 ,硒制剂诱导合成MT很可能是减轻阿霉素心脏毒性的保护机制之一。两种硒制剂比较使用富硒麦芽较亚硒酸钠更为安全 ,且不降低原已证实的亚硒酸钠对心肌的保护作用。
OBJECTIVE: To investigate the effect of selenium-containing preparations on the cardiotoxicity of doxorubicin induced by selenium and to compare selenium-enriched malt with inorganic selenium and selenium. Methods: Selenium was administered to mice at different time points to secrete blood serum. GOT and CK activity were measured routinely and GSH Px activity was measured by DTNB method. Methotrexate (MT) was measured by Hg Chelex ) Content and do heart pathology examination. Results: Both selenium-containing preparations could reduce the weight loss caused by doxorubicin, the increase of GOT and CK enzyme and the pathological damage of heart. The effect of selenium-enriched malt was slightly better than that of sodium selenite. Both also increase GSH Px enzymatic activity, inducing an increase in MT synthesis in the heart, and the overall effect of continuous use of the selenium preparation is superior to the first or later use of the preparation. Conclusion: In addition to elevated GSH Px, selenium-induced synthesis of MT may be one of the protective mechanisms that reduce the cardiotoxicity of doxorubicin. Comparison of two selenium preparations using selenium-enriched malt more than sodium selenite safer, and does not reduce the already proven sodium selenite myocardial protection.