目的 探讨B19病毒中国株NS基因的变异。方法 采用聚合酶链反应(PCR)技术,从中国再障患儿血清中扩增人细小病毒B19-XA17,XA18,XA20株的NS基因片段,进行核苷酸序列分析。结果 人细小病毒B19-XA17、XA18、XA20株的NS基因核苷酸序列被测定,测序结果与国外Au株基因核昔的序列比较,有4个核苷酸突变,并引起编码的4个氨基酸发生改变。结论 我国B19病毒NS基因序列与Au株相比有变异。 Objective To investigate the variation of NS gene in Chinese B19 strain. Methods The NS gene fragment of human parvovirus B19-XA17, XA18 and XA20 was amplified from the serum of children with aplastic anemia by Polymerase Chain Reaction (PCR), and the nucleotide sequence was analyzed. Results The nucleotide sequence of the NS gene of human parvovirus B19-XA17, XA18 and XA20 was determined. Compared with the sequence of the foreign Au gene, the nucleotide sequence of NS gene was sequenced. There were four nucleotide mutations and four amino acids changes happened. Conclusion The sequence of NS gene of B19 virus in our country is different from that of Au strain.