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目的:寻找胎鼠宫内脑损伤的有效预测指标。方法:比较正常对照组和缺氧组胎鼠脑组织形态学变化、脑组织中S100B蛋白和脑源性神经营养因子(BDNF)的表达及含量。结果:(1)脑组织形态学结果显示:缺氧组胎鼠大脑皮质和海马区细胞排列紊乱,细胞数目减少,结构模糊或消失,组织间质疏松水肿。(2)免疫组织化学染色结果显示:S100B蛋白主要在海马区的齿状回部位表达,BDNF主要在海马区和皮质区表达。缺氧组S100B蛋白及BDNF阳性细胞平均灰度值较正常对照组明显增强(189.90±31.66 vs 84.66±12.90,P=0.000;139.04±15.83 vs 74.86±14.08,P=0.000)。(3)酶联免疫吸附试验检测结果显示:缺氧组胎鼠脑组织中S100B蛋白、BDNF含量均明显高于正常对照组(3.17±1.07 ng/ml vs 2.25±1.20 ng/ml,P=0.006;152.08±36.29 pg/ml vs 128.21±43.43 pg/ml,P=0.039)。结论:孕鼠缺氧后可导致胎鼠脑损伤,脑组织中S100B蛋白、BDNF的测定可作为预测胎鼠宫内脑损伤的指标。
Objective: To find an effective predictor of fetal intrauterine brain injury. Methods: The morphological changes of brain tissue and the expression of S100B protein and brain-derived neurotrophic factor (BDNF) in normal and hypoxic groups were compared. Results: (1) The results of brain histomorphology showed that the cells in the cerebral cortex and hippocampus of hypoxic group were disordered, the number of cells was reduced, the structure was vague or disappeared, and the interstitial interstitial edema. (2) Immunohistochemical staining results showed that S100B protein was mainly expressed in the dentate gyrus of hippocampus, and BDNF was mainly expressed in hippocampus and cortex. The average gray value of S100B protein and BDNF positive cells in hypoxic group was significantly higher than that in normal control group (189.90 ± 31.66 vs 84.66 ± 12.90, P = 0.000; 139.04 ± 15.83 vs 74.86 ± 14.08, P = 0.000). (3) The results of enzyme-linked immunosorbent assay showed that the levels of S100B protein and BDNF in brain tissue of hypoxic group were significantly higher than those of normal control group (3.17 ± 1.07 ng / ml vs 2.25 ± 1.20 ng / ml, P = 0.006 ; 152.08 ± 36.29 pg / ml vs 128.21 ± 43.43 pg / ml, P = 0.039). CONCLUSION: The fetal rat brain injury can be induced by hypoxia in pregnant rats. The determination of S100B protein and BDNF in brain tissue may be used as an index to predict intrauterine brain injury in fetal rats.