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多年来检验药品的毒性绝大多数使用昆明种小鼠,但这种远交系小鼠个体差异较大。为提高试验准确性和实验结果可比性,华北制药厂动物场以昆明种为原种,经26代以上严格近亲繁殖,培育了近交系小鼠NCPC/4,遗传监测结果表明已符合近交系小鼠要求。该鼠具有生长速度快,繁殖能力强等特性。作者主要对NCPC/4小鼠进行免疫活性测定,包括T、B、Mφ、LAK、CTL、胸腺、脾和淋巴结的细胞免疫活性,血清溶菌酶含量,特异HgM的产生,PFC反应,IL-2活性及抗肿瘤、抗感染效应,并以C_(57)BL/6和NIH系小鼠作为对照。材料和方法一、动物NCPC/4(简称N_4)小鼠和C_(57)BL/6小鼠(简称C_(57))由华北制药厂实验动物场提供,2月龄,雌雄各半;2月龄NIH小鼠雌雄各半,由河北医学院动物室提供。
Over the years to test the toxicity of drugs the vast majority of Kunming mice, but this inbred mice larger individual differences. In order to improve the accuracy of the experiment and the comparability of experimental results, the animal farms in North China Pharmaceutical Factory used Kunming as the original species. After inbreeding for more than 26 generations, inbred mice NCPC / 4 were cultivated, and the genetic monitoring results showed that they were in conformity with inbred Department of mouse requirements. The mouse has a fast growth, reproductive ability and other characteristics. The authors mainly tested the immunocompetence of NCPC / 4 mice, including cellular immune activities of T, B, Mφ, LAK, CTL, thymus, spleen and lymph nodes, serum lysozyme content, production of specific HgM, PFC, IL- Activity and anti-tumor and anti-infective effects. C57BL / 6 and NIH mice were used as controls. MATERIALS AND METHODS 1. Animals NCPC / 4 (abbreviated as N_4) and C_ (57) BL / 6 mice (abbreviated as C_ (57)) were provided by experimental animals farm of North China Pharmaceutical Factory, 2 months old, The age of male and female NIH mice half, provided by the Hebei Medical College animal room.