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在不同激素水平及组合类型的培养基上,对枸杞不同发育期的果实进行胚乳离体培养,结果表明,青果期是枸杞胚乳培养的适宜期,黄果亦可作为取材对象,而红果效果较差。在枸杞胚乳诱导愈伤组织的培养基中加入一定浓度的细胞分裂素和生长素,产生的愈伤组织可直接形成芽体,使枸杞胚乳培养技术得到进一步改进。同时对胚乳苗根尖进行细胞染色体数目检查,染色体数目的变化范围在16~48条,多数细胞在16~24条,稳定的三倍体植株还未确定。
The fruit of different developmental stages was cultured in vitro on different hormone levels and combination types. The results showed that the green fruit stage was a suitable period for the culture of alfalfa endosperm, and the yellow fruit could also be used as the object, and the red fruit was more effective. difference. Adding cytokinins and auxin at a certain concentration to the callus induced by the endosperm of the endosperm induces the callus to directly form the buds, which further improves the culture technology of the endosperm. At the same time, the number of chromosomes in the root tip of the endosperm seedlings was examined. The number of chromosomes varied from 16 to 48, most cells were from 16 to 24, and stable triploid plants had not been determined.