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马铃薯在生长发育过程中受各种生物和非生物胁迫的影响，干旱是其中最常见和危害最严重的非生物胁迫因素之一，常常导致单产不高，总产不稳，严重影响着马铃薯产业的发展。本研究以改善马铃薯抗旱性为目的，采用PCR方法从拟南芥中克隆了转录因子DREB1 A基因和诱导型启动子 rd29 A。利用 DNA重组技术成功构建了诱导型启动子 rd29 A驱动转录因子DREB1 A基因和CaMV35 S启动子驱动Bar基因的双价植物表达载体 pBI121-rd29-BDR，并通过农杆菌介导法对马铃薯进行遗传转化，PPT筛选得到22株抗性苗，PCR和 RT-PCR检测证明DREB1 A基因已整合到陇薯10号马铃薯基因组中并在转基因植株中转录表达，有望提高转基因马铃薯的抗旱性。目前，作者正在进行转基因马铃薯的抗旱性分析研究。“,”Potato growth and productivity has been extremely affected by drought-stress.It is important to im-prove drought tolerance of potato to increase yield under stress conditions.To improve drought resistance of potato,the drought response transcription factor DREBIA gene and rd29A promoter were isolated from Arabi-dopsis thaliana by PCR.Using recombinant DNA technology,the bivalent plant expression vector pBI1 2 1-rd29-BDR,which contains two expression cassettes,rd29A-DREB1A-NOS and CaMV35S-Bar-NOS,was suc-cessfully constructed.Potato stems were used for the transformation.Excised stems (0.5-1.0 cm)were in-fected with Agrobacterium LBA4404/pBI121-rd29-BDR (OD600=0.5-0.8)and grown on a co-cultivation me-dium for 3 days in darkness,then placed on callus induction medium plates containing 2 mg/L PPT and 200 mg/L cefotaxime for callus formation.Twenty two resistant seedlings were obtained.The PCR and RT-PCR testing showed that DREB1A was not only integrated into the genome of the potato Longshu 10,but was also expressed in transgenic plants.To verify drought resistance of transgenic potato,analyses were carried out.