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研究目的:对两个品种金钱榕(var.deltoidea和var.angustifolia)叶片提取物抑制3T3-L1脂肪细胞脂肪沉积进行研究,为基于金钱榕的抗肥胖功能产品开发提供理论基础。研究方法:用甲醇和水分别对两个品种金钱榕的叶片进行提取,并分别分析了提取物中的总黄酮含量(TFC)和总酚含量(TPC)。通过MTT法确定提取物对3T3-L1脂肪细胞的最大无毒剂量(MNTD)。然后,通过油红O染色和吸光度测定来定性和定量研究提取物对抗脂肪细胞脂肪沉积的作用。重要结论:金钱榕两个品种deltoidea和angustifolia的甲醇提取物中的TFC含量分别为1.36和1.97 g QE/100 g DW,水提取物中的TPC含量分别为5.61和2.73 g GAE/100 g DW。金钱榕deltoidea品种的甲醇和水提取物对3T3-L1细胞的MNTD分别为(300.0±28.3)和(225.0±21.2)μg/ml,而angustifolia品种的MNTDs则较低(其甲醇和水提取物的MNTDs分别为(60.0±2.0)和(8.0±1.0)μg/ml)。金钱榕两个品种的甲醇提取物和angustifolia品种的水提取物在MNTD或1/2MNTD的浓度下,均具有显著抑制前脂肪细胞的成熟的作用。因此,根据其抑制成熟脂肪细胞形成的结果可以得出金钱榕叶片提取物具有潜在的抗肥胖效果。
OBJECTIVE: To study the inhibitory effect of leaf extracts of two species of Ficus variabilis (var.deltoidea and var. Angustifolia) on the fat deposition of 3T3-L1 adipocytes and to provide a theoretical basis for the development of anti-obesity functional products based on Ficus. Methods: The leaves of two species of Ficus microcarpa were extracted with methanol and water, and the content of total flavonoids (TFC) and total phenolics (TPC) in the extracts were analyzed. The maximum non-toxic dose (MNTD) of extracts on 3T3-L1 adipocytes was determined by MTT assay. The effect of the extracts on the anti-adipocyte fat deposition was then investigated qualitatively and quantitatively by oil red O staining and absorbance measurements. Important conclusions: The TFCs in the methanolic extracts of two species of Banyanaria deltoidea and Angustifolia were 1.36 and 1.97 g QE / 100 g DW, respectively. The TPC contents of the water extracts were 5.61 and 2.73 g GAE / 100 g DW, respectively. The MNTDs of methanol and water extracts of the fig. Deltoidea cultivars were (300.0 ± 28.3) and (225.0 ± 21.2) μg / ml for 3T3-L1 cells, while those of angustifolia cultivars were lower for MNTDs (methanol and water extracts MNTDs were (60.0 ± 2.0) and (8.0 ± 1.0) μg / ml, respectively). Both the methanolic extracts of both figs of Ficus microcarpa and the aqueous extract of angustifolia species had a significant inhibitory effect on the maturation of preadipocytes at concentrations of MNTD or ½MNTD. Therefore, based on the results of its inhibition of the formation of mature adipocytes, it can be concluded that the fig leaf extract has a potential anti-obesity effect.