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目的:探讨银杏叶提取物(EGb)通过诱导血红素加氧酶1(HO-1)的合成,保护小鼠急性肾脏缺血再灌注的损伤及其机制。方法:昆明种小鼠30只,随机分成假手术组、单纯缺血再灌注组、缺血再灌注+EGb组。测定缺血再灌注24h后小鼠血清肌酐与尿素氮值的变化,观察肾脏组织的病理学改变,并通过免疫组织化学方法观察小鼠肾脏血红素加氧酶1的表达。结果:与假手术组相比,单纯缺血再灌注组肾小管上皮细胞呈明显的缺血性改变,血清肌酐与尿素氮值水平均明显升高(P<0.01),肾脏血红素加氧酶1表达明显增强;缺血再灌注+EGb组与单纯缺血再灌注组相比,肾小管上皮细胞缺血性改变减轻,血清肌酐与尿素氮值水平均明显降低(P<0.01),肾脏血红素加氧酶1表达明显增强。结论:EGb通过诱导HO-1的合成,减轻小鼠急性肾缺血再灌注的损伤。
Objective: To investigate the protective effect of Ginkgo biloba extract (EGb) on the acute renal ischemia / reperfusion injury in mice induced by the induction of heme oxygenase 1 (HO-1). Methods: Thirty Kunming mice were randomly divided into sham-operated group, ischemia-reperfusion group and ischemia-reperfusion + EGb group. The changes of serum creatinine and urea nitrogen were measured 24h after ischemia-reperfusion, the pathological changes of kidney were observed, and the expression of heme oxygenase-1 in kidney of mice was observed by immunohistochemical method. Results: Compared with the sham-operated group, the renal tubular epithelial cells in ischemia-reperfusion group showed obvious ischemic changes, serum creatinine and urea nitrogen levels were significantly increased (P <0.01), renal heme oxygenase 1 was significantly increased in ischemic reperfusion + EGb group compared with those in ischemia-reperfusion group. The ischemic changes of renal tubular epithelial cells were alleviated, serum creatinine and urea nitrogen levels were significantly decreased (P <0.01) Superoxide 1 expression was significantly enhanced. Conclusion: EGb can reduce the injury of acute renal ischemia-reperfusion in mice by inducing HO-1 synthesis.