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目的 :观察脂多糖、白介素 - 6和肿瘤坏死因子α对大鼠星形胶质细胞组织因子活性表达的影响。方法 :培养Wistar乳鼠星形胶质细胞 ,细胞用抗神经胶质纤维酸性蛋白单克隆抗体确认。细胞冻融液组织因子活性检测采用一期血浆复钙时间法。结果 :脂多糖、白介素 - 6和肿瘤坏死因子α组星形胶质细胞表达组织因子活性明显高于对照组 (P <0 .0 5 )。脂多糖、白介素 - 6和肿瘤坏死因子α与三氟吡啦嗪 (TFP)或 1- (5 -异喹啉磺胺 ) - 3 -甲基哌嗪 (H7)联合运用 ,则它们的刺激作用受到明显抑制。结论 :脂多糖、白介素 - 6、肿瘤坏死因子能刺激星形胶质细胞表达组织因子活性 ,其机制可能与钙 /钙调素和蛋白激酶C途径有关。
Objective: To observe the effects of lipopolysaccharide, interleukin - 6 and tumor necrosis factor α on the expression of tissue factor in rat astrocyte. Methods: Wistar astrocytes were cultured and the cells were confirmed by anti-glial fibrillary acidic protein monoclonal antibody. Cell freeze-thaw fluid tissue factor activity test using a period of plasma calcium complex time method. Results: Tissue factor expression in astrocytes of lipopolysaccharide, interleukin - 6 and tumor necrosis factor α group was significantly higher than that of control group (P <0.05). Lipopolysaccharide, interleukin-6, and tumor necrosis factor alpha, in combination with trifluoperazine (TFP) or 1- (5-isoquinoline sulfanilamide) -methyl piperazine (H7) Obvious inhibition. CONCLUSION: Lipopolysaccharide, interleukin - 6 and tumor necrosis factor can stimulate the activity of tissue factor in astrocytes. The mechanism may be related to calcium / calmodulin and protein kinase C pathway.