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AIM:To assess the inhibitory effect of Huangqi Zhechongdecoction on hepatic fibrosis in rats induced by CCl_4 plusalcohol and high fat low protein diet.METHODS:Male SD rats were randomly divided into hepaticfibrosis model group,control group and 3 treatment groupsconsisting of 12 rats in each group.Except for the normalcontrol group,all the rats were subcutaneously injected withCCl_4 at a dosage of 3 mL/kg.In 3 treated groups,eitherhigh-dose group(9 mL/kg),or medium-dose group(6 mL/kg),or low-dose group(3 mL/kg)was daily garaged with HuangqiZhechong decoction,and saline vehicle was given to modeland normal control rats.Enzyme-linked immunosorbent assay(ELISA)and biochemical examinations were used todetermine the changes of alanine aminotransferase(ALT),aspartate aminotransferase(AST),hyaluronic acid(HA),laminin(LN),type-Ⅲ-procollagen-N-peptide(PIIIP),andtype Ⅳ collagen content in serum,and hydroxyproline(Hyp)content in liver after sacrificing the rats.Pathologic changes,particularly fibrosis were examined by hematoxylin and eosin(HE)and Van Gieson staining.RESULTS:Compared with the model control group;serumALT,AST,HA,LN,PⅢP and type Ⅳ collagen levels droppedmarkedly in Huangqi Zhechong decoction groups,especiallyin the medium-dose Huangqi Zhechong decoction group(1954+576 U/L vs 759±380 U/L,2 735±786 U/L vs1259±829 U/L,42.74±7.04 ng/mL vs 20.68±5.85 ng/mL,31.62±5.84 ng/mL vs 14.87±1.45 ng/mL,3.26±0.69 ng/mLvs 1.47±0.46 ng/mL,77.68±20.23 ng/mL vs 25.64±4.68ng/mL,respectively)(P<0.05).The Hyp content in liver tissuewas also markedly decreased(26.47±11.24 mg/mgprot vs9.89±3.74 mg/mgprot)(P<0.01).Moreover,the stage ofthe rat liver fibrosis in Huangqi Zhechong decoction groupswas lower than that in model group,and more dramaticdrop was observed in medium-dose Huangqi Zhechongdecoction group(P<0.01).CONCLUSION:Huangqi Zhechong decoction can inhibithepatic fibrosis resulted from chronic liver injure,retard thedevelopment of cirrhosis,and notably ameliorate the liver function.It may be a safe and effective therapeutic drug forpatients with fibrosis.
AIM:To assess the inhibitory effect of Huangqi Zhechongdecoction on hepatic fibrosis in ratsinduced by CCl_4 plusalcohol and high fat low protein diet.METHODS:Male SD rats were randomly divided into hepaticfibrosis model group,control group and 3 treatment groupsconsisting of 12 rats in each group.Except for the normalcontrol group,all the rats were subcutaneously injected withCCl_4 at a dosage of 3 mL/kg.In 3 treated groups,eitherhigh-dose group(9 mL/kg),or medium-dose group(6 mL/kg ), or low-dose group(3 mL/kg)was daily garaged with HuangqiZhechong decoction,and saline vehicle was given to modeland normal control rats.Enzyme-linked immunosorbent assay(ELISA)and biochemical examinations were used todetermine the changes of alanine aminotransferase (ALT), aspartate aminotransferase (AST), hyaluronic acid (HA), laminin (LN), type-III-procollagen-N-peptide (PIIIP), and type IV collagen content in serum, and hydroxyproline (Hyp) content in liver after Sacrificing the rats.Pathologic changes,particularl y fibrosis were examined by hematoxylin and eosin(HE)and Van Gieson staining.RESULTS:Compared with the model control group;serumALT,AST,HA,LN,PIIIP and type IV collagen levels droppedmarkedly in Huangqi Zhechong decoction groups,especiallyin the medium- Dose Huangqi Zhechong decoction group (1954+576 U/L vs 759±380 U/L, 2 735±786 U/L vs1259±829 U/L, 42.74±7.04 ng/mL vs 20.68±5.85 ng/mL, 31.62± 5.84 ng/mL vs 14.87±1.45 ng/mL, 3.26±0.69 ng/mL vs 1.47±0.46 ng/mL, 77.68±20.23 ng/mL vs 25.64±4.68 ng/mL, respectively (P<0.05). The Hyp content In liver tissue was also markedly decreased (26.47±11.24 mg/mgprot vs 9.89±3.74 mg/mg prot) (P<0.01). Moreover, the stage of the rat liver fibrosis in Huangqi Zhechong decoction groupswas lower than that in model group,and more Dramaticdrop was observed in medium-dose Huangqi Zhechongdecoction group(P<0.01).CONCLUSION:Huangqi Zhechong decoction can inhibithepatic fibrosis caused from chronic liver injure,retard thedevelopment of cirrhosis, and notably amelio Rate the liver function.It may be a safe and Effective therapeutic drug forpatients with fibrosis.