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为了快速准确地配型肾移植供受体的ＨＬＡ型别，对ＨＬＡ分型试验的影响因素进行了探讨。结果显示：采用玻璃珠脱纤维抗凝血标本，可以获得比肝素抗凝法更高纯度的淋巴细胞，并能大大地降低细胞悬液中血小板的污染。加样时采用软加法可避免相邻孔间反应的相互污染。ＨＬＡ抗体和补体相互间能弥补因对方效价下降而引起的试验敏感性降低。使用淋巴细胞分离液分离细胞时应根据不同的试剂生产厂家而区别对待细胞分离时的离心时间。
In order to quickly and accurately match the type of HLA donor kidney recipients, HLA typing test factors were discussed. The results showed that the use of glass beads defibrillation anticoagulant samples can be obtained than the heparin anticoagulant purity of lymphocytes, and can greatly reduce the cell suspension of platelet contamination. Addition of soft addition method to avoid the interaction between adjacent holes mutual pollution. HLA antibodies and complement each other to make up for each other due to the decline in potency and reduce the test sensitivity. When using lymphocyte separation fluid to separate cells, the centrifugation time at the time of cell separation should be differentiated according to different reagent manufacturers.